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991.
The use of grain protein deviation for identifying wheat cultivars with high grain protein concentration and yield 总被引:2,自引:0,他引:2
James M. Monaghan John W. Snape A. Jan S. Chojecki Peter S. Kettlewell 《Euphytica》2001,122(2):309-317
The relationship between grain protein concentration and grain yield in different cultivars of winter wheat was examined in
a series of field experiments carried out over three years, in which 13, 12 and 8 cultivars were studied in each year, respectively.
The plants were grown at sites located in Shropshire, west-central England, in years 1 and 2, and at three other locations
in eastern England in year 3. Above ground plant samples were collected at an thesis and again at maturity, when they were
separated into grain and straw, and analysed for dry matter and N content. Analysis of residuals from regression of grain
protein concentration on grain yield (grain protein deviation, GPD) showed that some cultivars had a higher grain protein
concentration than was predicted from grain yield alone. It was deduced that the capacity to accumulate a higher grain protein
concentration than predicted from grain yield is under genetic control and thus may be improved through breeding. Other factors
(weight of N accumulated in the biomass at anthesis, weight of N accumulated in the biomass between anthesis and maturity
and the concentration of N remaining in the straw at maturity) were added step-wise into the regression to enable statistical
analysis of their relative contributions to grain protein. High GPD may be achieved through increased N accumulation after
anthesis, combined with efficient re-translocation of vegetative N reserves. The use of GPD provides a selection criteria
in wheat breeding programs to screen for increased grain protein concentration without a concurrent grain yield reduction.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
992.
Two male-sterile lines, KalashreeA and PadminiA, with a Miz.21 cytoplasm source were developed through indica/indica hybridization followed by repeated backcrossing with their respective recurrent male parents (Kalashree and Padmini) up to the BC6 generation. These two cytoplasmic male-sterile lines are suitable for use in the development of hybrids for lowland situations owing to their intermediate to semi-tall stature, late flowering duration, good grain quality and easy fertility restoration ability. 相似文献
993.
Summary Better understanding of the physiological and genetic basis of wheat grain protein will contribute to breeding efforts for this characteristic. This study provides information about plant protein distribution in high and low grain protein winter wheats (Triticum aestivum L.) at different growth stages and its relation to grain protein. Field experiments involved two winter wheats with high grain protein, Redwin and Lancota, and two with low grain protein, Centurk and Brule in two years. Protein content in the head, the upper three leaves, the first and second leaf, and the peduncle were estimated with Near Infrared Reflectance Spectrophotometer (NIR) at five growth stages. High protein cultivars had higher leaf protein at ripe and higher protein content in the heads at most growth stages than low grain protein cultivars. High protein cultivars had lower protein content in the peduncle than low protein cultivars at ripe. Correlation coefficients between plant-part protein and grain protein ranged from 0.48 to 0.87 for the heads, from –0.45 to –0.79 for the peduncle, and from 0.55 to 0.84 for the leaves. A combination of head, peduncle, and first leaf protein at heading was significantly related to grain protein (R2=0.71). Indirect selection for head, peduncle, and first leaf (flag leaf) protein at heading should result in increased grain protein. Recurrent selection for increased grain protein, with parent selectionbefore anthesis and hybridization should be successful. 相似文献
994.
995.
Karyotypic and electrophoretic studies on taro and its origin 总被引:4,自引:0,他引:4
Summary Karyotypes and electrophoretic pattern of 15 strains of taro were studied. Strains collected from the northeast India hill state, Meghalaya were diploids and triploids whereas those from the plains of south India were diploids and of north India was a triploid. The diploids had 2n=28 and triploids showed 2n=42 chromosomes. The wild taro had the most asymmetrical karyotype. The protein content varied from 4.2 to 11.4 mg/g dry wt. The maximum protein content was found in a triploid strain 8 (11.4 mg/g dry wt) and minimum in the wild taro (4.2 mg/g dry wt). The number of protein bands was 7 in the wild taro (diploid) and 12 in one of the cultivated triploid strain. Meghalaya strains showed great variation with respect to leaf size and tuber shape and size. All the strains have diverged at morphological, karyotypic and genotypic levels. It is suggested that taro might have originated in the north-eastern India. 相似文献
996.
冬小麦体细胞无性系种子蛋白质含量的遗传 总被引:3,自引:0,他引:3
从同一单倍体的幼穗、成熟胚、继代花药愈伤组织和种子植株幼穗获得的无性系,其蛋白质含量的变幅很大,分别为12.14% ̄21.33%、13.98% ̄20.51%,13.82% ̄17.97%和16.62% ̄22.16%。无性系蛋白质含量的变异发生在早代。获得的高蛋白特性可以遗传。已从单倍体幼穗无性系获得5个蛋白质含量高(19%以上)的冬小麦新品系。 相似文献
997.
998.
优化的VHb基因和GFMcryIA基因构建的双价基因在烟草中的表达 总被引:1,自引:0,他引:1
利用人工合成的、密码子优化后的透明颤菌血红蛋白(VHb)基因与人工合成的GFMcryIA基因构建成双价基因植物高效表达载体。通过根癌农杆菌介导转化烟草,获得了36株卡那霉素抗性植株。经转基因植株的PCR及Southern blot检测,证实了双价基因在35株烟草基因组中的整合。Western blot检测证实了VHb基因的表达,杀虫实验证实GFMcryIA基因也表达出活性毒蛋白。且转基因烟草平均每株干重比非转基因烟草植株高7%。本研究结果表明转基因育种技术在培育出既高产又具抗虫性的作物或经济作物新品种方面具有良好的应用前景。 相似文献
999.
Expression of nucleolus, endosperm storage proteins and disease resistance in an amphiploid between Aegilops tauschii and Secale silvestre 总被引:1,自引:0,他引:1
Cytology and gene expression of an amphiploid between Aegilops tauschiiL., native to China, and Secale silvestre L. were studied to reveal the genomic interaction between the donor species. High frequencies of aneuploids were observed
in the progenies of the amphiploid, indicating its cytological instability. Feulgen staining and Giemsa-C banding showed that
only the nucleolar organizing region from chromosome 5D of Ae. tauschii existed in the amphiploid (2n = 28). The nucleolus of S. silvestre was not observed. Endosperm storage protein electrophoresis indicated most gliadin and glutenin genes from both parents were
expressed in the endosperm of the amphiploid. When inoculated by wheat stripe rust and powdery mildew isolates,the amphiploid
did not express the resistance from its Secale parent,suggesting the presence of disease resistance suppressor(s) in the D genome of Ae. tauschii as well as nucleolar organizer suppressors.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
1000.
小麦黄矮病新抗源中4,中5的选育及应用研究 总被引:8,自引:0,他引:8
中4、中5是用经过连续3年系统选育的天蓝偃麦草作父本,地理远缘的小麦品种克强与南大2419杂交的F_5后代材料作母本,通过两者杂交,并采取延长生育法等技术克服F_1不育性,经过连续9年选育而成.对小麦黄矮病高抗,对条、叶、秆三种锈病的多种生理小种均表现免疫至高抗,还具有高蛋白(含量17.08%、17.13%);高赖氨酸(含量为0.483%、0.50%)等特点,是人工合成的优异的小麦多抗、优质资源.用中4、中5与普通小麦杂交,已成功地将抗黄矮病基因转移到普通小麦,育成陕麦8007、陕麦8124、忻4070、忻4079、中1001等品种(系). 相似文献